Journal: Journal of thoracic disease

Article Title: Comparison of next-generation sequencing and immunohistochemistry analysis for targeted therapy-related genomic status in lung cancer patients.

doi: 10.21037/jtd.2019.12.25

Figure Lengend Snippet: Figure 3 Results of IHC staining and NGS for the EGFR E746-A750Del, L858R, ALK, and ROS1 detection. “n” in the figure indicates the

Article Snippet: IHC staining of tumor tissues was performed on 4-μm sections using the standard procedure and primary monoclonal antibodies against EGFR L858R (clone: 43B2, 1:200, Cell Signaling Technology), EGFR E746-A750del (clone: 6B6, 1:200, Cell Signaling Technology, Danvers, MA), ALK (clone: D5F3, 1:200, Ventana, Tucson, AZ), and ROS1 (clone: D4D6, 1:200, Cell Signaling Technology).

Techniques: Immunohistochemistry

Journal: PLoS ONE

Article Title: c-Src and Neural Wiskott-Aldrich Syndrome Protein (N-WASP) Promote Low Oxygen-Induced Accelerated Brain Invasion by Gliomas

doi: 10.1371/journal.pone.0075436

Figure Lengend Snippet: A list of the molecules.

Article Snippet: Immunodetection was performed using the following primary antibodies from Cell Signaling (Danvers, MA, USA): AKT, p-AKT (S473), -Catenin, Cofilin, p-Cofilin (Ser3), Cortactin, p-Cortactin (Tyr421), EGFR, p-EGFR (Tyr845), p-EGFR (Tyr992), p-EGFR (Tyr1068), FAK, p-FAK (Tyr397), p-FAK (Tyr576/577), HIF-1 , p44/42MAPK, p-p44/42 MAPK(Thr202/Tyr204), MyPT1, p-MYPT1(Thr853), Notch1, Cleaved Notch1, PAK1/2/3, p-PAK1(Thr423)/PAK2(Thr402), P53, PP5, Src, p-Src(Tyr416), p-Src (Tyr527), Stat3, p-Stat3(Tyr705), and GAPDH.

Techniques:

Journal: Cell chemical biology

Article Title: Aptamers as reversible sorting ligands for preparation of cells in their native state

doi: 10.1016/j.chembiol.2019.12.004

Figure Lengend Snippet: (A) Overview of AF488-SA-E07 aptamer-staining and FACS sorting of EGFR(+) cells followed by antidote treatment to remove the E07 stain and leave the cells ligand-free, reversing the EGFR antagonist effects of the E07 aptamer and restoring native signaling. EGFR(+) A431 cells were stained with AF488-SA-E07 and sorted for E07/AF488+ signal generating a pool of cells with aptamer-neutralized EGFR signaling. Destaining with antidote mA9 generated a pool of ligand-free unlabeled cells with restored EGFR signaling pathways. (B–D) Quantitative western blots of A431 cells probed for both phosphorylated EGFR (pEGFR; red) and total EGFR (green) after stimulation with EGF (indicated by “+EGF”). Cells were sorted for EGFR expression after staining with either 500nM AF488-SA-E07 or with a 1:50 dilution of the EGFR-antagonizing antibody (D1D4J). Cells alone or cells stained with the control C36 aptamer were mock sorted by running them through the cell sorter. While EGF stimulated EGFR phosphorylation in both unstained cells and cells stained with the control C36 aptamer, incubating/staining both unsorted (C) and sorted (D) cells with either AF488-SA-E07 or an EGFR-antagonizing antibody impedes stimulation of EGFR by EGF, indicating compromised receptor function. Subsequent treatment with mA9 (5 mM) for 5 min at 37°C efficiently removed AF488-SA-E07 to restore EGFR stimulation to native wild type levels. In contrast, irreversibly bound antibody permanently inhibited stimulation of EGFFR by EGF (n ≥ 3). One-way ANOVAs followed by Tukey-Kramer post-hoc tests were used to determine significance as indicated by * (p = 0.012) and **** (p < 0.0001). Data are mean ± SEM. See also Figure S6.

Article Snippet: EGFR Antibody Reversal with the EGFR extracellular domain Antibody staining solutions contained a 1:50 dilution of PE-labeled EGFR neutralizing antibody D1D4J (Cell Signaling Technologies) in Media+.

Techniques: Staining, Generated, Western Blot, Expressing

Journal: Cell chemical biology

Article Title: Aptamers as reversible sorting ligands for preparation of cells in their native state

doi: 10.1016/j.chembiol.2019.12.004

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: EGFR Antibody Reversal with the EGFR extracellular domain Antibody staining solutions contained a 1:50 dilution of PE-labeled EGFR neutralizing antibody D1D4J (Cell Signaling Technologies) in Media+.

Techniques: Recombinant, Modification, Lysis, Staining, Cell Culture, Synthesized, Software

Journal: Cell Death & Disease

Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers

doi: 10.1038/s41419-024-06916-y

Figure Lengend Snippet:

Article Snippet: Phospho-EGFR , 1:1000 , Cell Signaling, 1068S.

Techniques:

Journal: Cell Death & Disease

Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers

doi: 10.1038/s41419-024-06916-y

Figure Lengend Snippet:

Article Snippet: Phospho-EGFR , 1:1000 , Cell Signaling, 1068S.

Techniques:

Journal: Oncotarget

Article Title: Efficacy of aerosol therapy of lung cancer correlates with EGFR paralysis induced by AvidinOX-anchored biotinylated Cetuximab

doi:

Figure Lengend Snippet: Fluorescence imaging by High Content Screening (HCS) Operetta of A549 cells, with or without AvidinOX conjugation (100 μg/mL), incubated with 5 μg/mL CF488-labelled bMabs (blue). At indicated time, cells were washed, fixed and stained for the detection of EGFR by AF555-labeled anti-EGFR Mab (D38B1) (red). Draq5 dye staining of nucleus and cytoplasm (yellow). Violet is the result of blue and red dye co-localization in the merged images. A, bCet, 2 h cultivation. B, bCet, bPan or bRit, 30 min or 30 min contact and 24 h cultivation. C, Unlabelled bCet 30 min contact and 24 h cultivation. Staining of lysosomes by LysoTracker (light blue) and staining of EGFR as before. Hoechst dye staining of nuclei (yellow). All panels: representative picture of at least 5 fields of triplicate wells. Magnification 60X.

Article Snippet: EGFR was detected by AF555-conjugated rabbit anti-EGFR (D38B1) (Cell Signaling), added after cell fixation with 4% paraformaldehyde in PBS, permeabilization with PBS 0.2%Tween-20 (PBS-T) and blocking with 2% BSA in PBS-T.

Techniques: Fluorescence, Imaging, High Content Screening, Conjugation Assay, Incubation, Staining, Labeling